42. Joshi,P.U.#, Turpeinen, D.G. #, Schroeder, M.*, Jones, B.*, Lyons, A.*, Kriz, S.A.#, Khaksari, M., O’Hagan, D., Nikam, S., and Heldt, C.L.+ Osmolyte Enhanced Aqueous Two-Phase System for Virus Purification. Under review.

41. Turpeinen, D.G. #, Joshi,P.U.#, Kriz, S.A.#, Kaur, S., Nold, N.M.#, O’Hagen, D., Nikam, S., Massoud, H., and Heldt, C.L.+ (2021) Continuous Downstream Purification of Viral Products Using an Aqueous Two-Phase System. Separation Science and Technology. Accepted.

40. Meingast, C.L. #, Joshi, P.U. #, Turpeinen, D.G. #, Xu, X., Hostein, M., Feroz, H., Ranjan, S., Ghose, S., Li, Z.J., and Heldt, C.L.+. (2021) Physiochemical Properties of Enveloped Viruses and Arginine Dictate Inactivation. Biotechnology Journal. Accepted.

39. Mi, X.#, Blocher McTigue, W.C., Joshi,P.U.#, Bunker,M.K.*, Heldt,C.L.+ and Perry, S.L.+ (2020) Stabilization of Viruses: A Stepping Stone toward Thermostable Vaccines. Biomaterials Science. 8, 7082. (invited paper) doi: 10.1039/d0bm01433h. Highlighted as one of Biomaterials Science Most Popular 2020 articles.

38. Mi, X.#, Albukhari, S.M., Heldt, C.L.+, and Heiden, P.A.+ (2020) Virus and chlorine adsorption onto guanidine modified cellulose nanofibers using covalent and hydrogen bonding. Carbohydrate Research. 498, 108153. doi: 10.1016/j.carres.2020.108153

37. Kord Forooshani, P., Pinnaratip, R., Polega, E., Tyo, A., Pearson, E. #, Liu, B., Folayan, T.O., Pan, L., Rajachar, R., Heldt, C.L.  and Lee, B.+ (2020) Hydroxyl Radical Generation Through the Fenton-Like Reaction of Hematin- and Catechol-Functionalized Microgels. Chemistry of Materials. 32 (19) 8182-8194. doi: 10.1021/acs.chemmater.0c01551

36. Mi, X.# and Heldt, C.L.+ (2020) Single-particle chemical force microscopy to characterize virus surface chemistry. BioTechniques. 69 (5), 363-370. doi: 10.2144/btn-2020-0085

35. Joonaki, E.+ Hassanpouryouzband, A., Heldt, C.L., and Areo, O.# (2020) Surface Chemistry Can Unlock Drivers of Surface Stability of SARS-CoV-2 onto the Surfaces in Variety of Environmental Conditions. Chem. 6(9), 2135-2146. doi: 10.1016/j.chempr.2020.08.001

34. Habibi, S., Joshi, P.U. #, Mi, X. #, Heldt, C.L., and  Minerick, A.M.+ (2020) Changes in membrane dielectric properties of porcine kidney cells provide insight into the antiviral activity of glycine. Langmuir. 36 (29), 8344-8356. doi: 10.1021/acs.langmuir.0c00175 

33. Mi, X., Bromley, E.K.*, Joshi, P.U., Long, F., and Heldt, C.L.+ (2020) Virus isoelectric point determination using single-particle chemical force microscopy. Langmuir. In press. doi: 10.1021/acs.langmuir.9b03070.

32. Meingast, C.L., and Heldt, C.L.+. (2020) Exploring the Mechanism of Arginine Enveloped Virus Inactivation. Biotechnol Prog. 36(2), e2931. doi: 10.1002/btpr.2931.

31. Joshi, P.U., Weiss, M.*, Corbin-Escalante, G.*, Schroeder, M.*,and Heldt, C.L.+. (2019) Tie Line Framework to Optimize Non-Enveloped Virus Recovery in Aqueous Two-Phase Systems. Journal of Chromatography B. 1126-1127. 121744.

30. Mi, X., Lucier, E.M.*, Turpeinen, D. G., Yeo, E.L.L, Kah, J.C.Y.+, and Heldt, C.L.+. (2019) Mannitol-induced gold nanoparticle aggregation for the ligand-free detection of viral particles. Analyst. 144: 5486-5496.

29. Heldt,C.L.+ (2019) Scalable method utilizing low pH for DNA removal in the harvest of recombinant adeno-associated virus vectors. Journal of Chromatography B. 1124. 173-179.

28. Meng, H., Forooshani, P.K, Joshi, P.U., Osborne, J., Mi, X., Meingast, C., Kelley, J., Narkar, A.R., He, W., Frost, M.C., Heldt, C.L., and Lee, B.P.+ (2019) Biomimetic Recyclable Microgels for On Demand Generation of Hydrogen Peroxide and Antipathogenic Application. Acta Biomaterialia. 83(1). 109-118.

27. Heldt,C.L.+, Smith, E., Cunningham, H.*, and Miller, C.* (2018) Mentoring Students in Research and Communication Across the Ocean. Perspectives in Undergraduate Research and Mentoring. PURM 7.1.

26. Heldt,C.L.+, Saksule, A., Joshi, P.U., and Ghafarian., M. (2018) A Generalized Purification Step for Viral Particles Using Mannitol Flocculation. Biotechnology Progress Accepted.

25. Heldt,C.L.+, Nwamba, C., Radecki, B., Covard, D.*, and Czeka, S.* (2018) Environmental research to engage community college students. ASEE North Central Conference Proceedings. Spring.

24. Heldt,C.L.+, Zahid,A.*, Vijayaragavan,K.S. and Mi, X. (2017) Experimental and Computational Surface Hydrophobicity Analysis of a Non-enveloped Virus and Proteins. Colloids and Surfaces B. 153, 77-84.

23. Saha, D.+, Heldt, C. L., Gencoglu, M.F., Vijayaragavan,K.S., Chen, J., and Saksule,A. (2016) A Study on the Cytotoxicity of Carbon-based Materials.Materials Science and Engineering C. 68, 101-108.

22. Pearce, J.M.+, Anzalone, N.C.*, and Heldt, C. L. (2016) Open-source Wax RepRap 3-D Printer for Rapid Prototyping Paper-Based Microfluidics. Journal of Laboratory Automation.  21(4), 510-516.

21. Heldt, C. L.+, Bank, A.*, Turpeinen, D.*, and King, J.A. (2016)  Translating University Biosensor Research to a High School Laboratory Experience.  Chemical Engineering Education. 50 (1), 70-75.

20. Gencoglu, M. F. and Heldt, C. L.+ (2015) Enveloped Virus Flocculation and Removal in Osmolyte Solutions. Journal of Biotechnology.  206, 8-11. doi:10.1016/j.jbiotec.2015.03.030

19. Gencoglu, M. F., Spurri, A.*, Franko, M.*, Chen, J., Hansley, D. K., Heldt, C. L.+, Saha, D.+ (2014) Biocompatibility of Soft-templated Mesoporous Carbons. ACS Applied Materials & Interfaces 6, 15068-15077.  DOI: 10.1021/am503076u

18. Vijayaragavan, K. S., Zahid, A.*, Young, J.W.* and Heldt, C. L.+ (2014).  Separation of porcine parvovirus from bovine serum albumin using PEG-salt aqueous two-phase system. Journal of Chromatography B 967, 118-126.  doi:10.1016/j.jchromb.2014.07.025

17.  Mi, X and Heldt, C. L.+ (2014) Adsorption of a non-enveloped mammalian virus to functionalized nanofibers. Colloids and Surfaces B: Biointerfaces 121, 319-324.  doi:10.1016/j.colsurfb.2014.06.007

16. Gencoglu, M. F., Pearson, E.* and Heldt, C. L.+ (2014). Porcine Parvovirus Flocculation and Removal in the Presence of Protecting Osmolytes.  Journal of Biotechnology 186, 83-90.  doi:10.1016/j.jbiotec.2014.06.011

15.  Xie, H., Tewari, R., Fukushima, H., Narendra, J., Heldt, C. L., King, J. A. and Minerick, A. R.+ (2014). Development of a 3D graphene electrode dielectrophoresis device.  Journal of Visualized Experiments. 88, e51696.

14. Mi, X., Vijayaragavan, K. S. and Heldt, C. L.+ (2014). Virus Adsorption of Water-Stable Quaternized Chitosan Nanofibers.  Carbohydrate Research, 387: 24-29.  doi:10.1016/j.carres.2014.01.017

13.  Bai, B., Mi, X., Xiang, X., Heiden, P. A. and Heldt, C. L.+ (2013). Non-Enveloped Virus Reduction with Quaternized Chitosan Nanofibers Containing Graphene. Carbohydrate Research 380, 137-142. doi:10.1016/j.carres.2013.08.020

12.  Tafur, M. F., Vijayaragavan, K. S. and Heldt, C. L.+ (2013). Reduction of Porcine Parvovirus Infectivity in the Presence of Protecting Osmolytes. Antiviral Research 99, 27-33.  doi:10.1016/j.antiviral.2013.04.019

11.  Heldt, C. L.+, Sieloff, A. M.*, Merillat, J. P.*, Minerick, A. R., King, J. A., Perger, W. F., Fukushima, H. and Narendra, J. (2013). Stacked graphene nanoplatelet paper sensor for protein detection. Sensors and Actuators B: Chemical 181, 92-98.  doi:10.1016/j.snb.2013.01.041

10. Heldt, C. L.+ (2012). Peer Evaluation in Chemical Engineering Capstone Design Via Wikis. Chemical Engineering Education 46, 189-195.


Postdoctoral Publications of C.L. Heldt

9. Zonca, M. R., Yune, P. S., Heldt, C. L., Belfort, G. and Xie, Y. (2013). High-Throughput Screening of Substrate Chemistry for Embryonic Stem Cell Attachment, Expansion, and Maintaining Pluripotency. Macromolecular Bioscience 13, 177-190.

8. Sorci, M., Gu, M., Heldt, C. L., Grafeld, E.* and Belfort, G. (2013). A multi-dimensional approach for fractionating proteins using charged membranes. Biotechnology and Bioengineering 110, 1704-1713.

7. Heldt, C. L., Kurouski, D., Sorci, M., Grafeld, E.*, Lednev, I. K. and Belfort, G. (2011). Isolating toxic insulin amyloid oligomers that lack b-sheets and have wide pH stability. Biophysical Journal 100, 2792-2800.

6. Heldt, C. L., Zhang, S.* and Belfort, G. (2011). Asymmetric Amyloid Fibril Elongation: A New Perspective on a Symmetric World. Proteins 79, 92-98. Highlighted in C&E News, Nov 2010.

5. Heldt, C. L., Sorci, M., Posada, D., Hirsa, A. and Belfort, G. (2011). Detection and Reduction of Microaggregates in Insulin Preparations. Biotechnology and Bioengineering 108, 237-241.


Ph.D. Publications of C.L. Heldt

4. Heldt, C. L., Gurgel, P. V., Jaykus, L. A. and Carbonell, R. G. (2012). Porcine parvovirus removal using trimer and biased hexamer peptides. Biotechnology Journal 7, 558-565.

3. Heldt, C. L., Gurgel, P. V., Jaykus, L. A. and Carbonell, R. G. (2009). Influence of Peptide Ligand Surface Density and Ethylene Oxide Spacer Arm on the Capture of Porcine Parvovirus. Biotechnology Progress 25, 1411-1418.

2. Heldt, C. L., Gurgel, P. V., Jaykus, L. A. and Carbonell, R. G. (2008). Identification of Trimeric Peptides That Bind Porcine Parvovirus from Mixtures Containing Human Blood Plasma. Biotechnology Progress 24, 554-560.

1. Heldt, C. L., Hernandez, R., Mudiganti, U., Gurgel, P. V., Brown, D. T. and Carbonell, R. G. (2006). A colorimetric assay for viral agents that produce cytopathic effects. Journal of Virological Methods 135, 56-65.

*undergraduate, +corresponding author, #graduate student advised by C. Heldt